Figure 2

EGFR, MUC4, and MMP9 localization in GBM. (A) Example of one GBM case with extended cytoplasmic EGFR expression. Note the total absence of EGFR-expressing cells in the proliferative microvasculature (white arrows). Magnification. 40 ×. (B) Example of one GBM case displaying dispersed cytoplasmic expression of MUC4. MUC4 is expressed in the endothelial wall (inner layer) of GBM microvasculature (red arrows) and in neoplastic cells, but not in the outer layers (white arrows and black dotted lines). Magnification, 20 ×. (C) Example of one GBM case displaying dispersed cytoplasmic expression of MMP9 in neoplastic areas showing pleomorphism. Magnification, 20 ×. (D, E) Representative images showing specific and restricted expression of MMP9 in cells located inside vessels, resembling immune cells, in one GBM patient; the surrounding neoplastic cells are negative for MMP9. Magnification, 40 ×. (F) Representative image showing MMP9 strong expression in microvascular proliferative structure (glomeruli-shaped) in one GBM patient. Purple arrows indicate MMP9-expressing cells; red blood cells (RBC) localization is indicated by red arrows Magnification, 40 ×. (G) Example of a GBM area showing expression of MUC4 in cancer cells. Magnification, 20 ×. (H, I) Images showing MUC4 expression in endothelial walls of microvascular proliferative areas (purple arrows). Two examples (from two different GBM patients) are shown. RBC red blood cells. Magnification, 20 ×.