Table 1 IC₅₀ values for JMJD5^FL inhibitors that likely bind in the 2OG cosubstrate pocket. A full-length JMJD5 construct (aa 1–416, JMJD5^FL) was used in the inhibition assays. IC₅₀ values were determined using MALDI-TOF-based hydroxylation assays with 10 µM JMJD5^FL, 100 µM Fe(II), 400 µM ascorbate, 50 µM 2OG and 100 µM substrate RpS6_129-144 (VPRRLGPKRASRIRKL) in the presence of varied concentrations of inhibitors (0–10 mM). Values represent mean (n = 3) ± SD.

From: Structural analysis of the 2-oxoglutarate binding site of the circadian rhythm linked oxygenase JMJD5