Figure 2 | Scientific Reports

Figure 2

From: Selection of red fluorescent protein for genetic labeling of mitochondria and intercellular transfer of viable mitochondria

Figure 2

Aggregate formation of mitochondria targeted mCherry and DsRed proteins. (A) Representative images of iAECs transduced with each mitochondria-targeted protein or uninfected control stained with MitoTracker and Hoechst. Merged images of each color are shown. Blue: Hoechst 33342. For DsRed, mCherry, TurboRFP mKOκ and uninfected control, green: MitoTracker Green, red: each fluorescent protein. For EGFP, green: EGFP, red: MitoTracker Orange. Scale bar = 10 µm. (B) Distribution of the colocalization coefficients of fluorescent proteins and MitoTracker of the cells. Each group included at least 80 cells from 7 optical fields. (C) Proportion of cells with aggregates in the mitochondria-targeted fluorescent protein-transduced cell population. Data are shown as the mean ± SD, n = 3 biological replicates. Each replicate included at least 80 cells from 7 optical fields. **p < 0.01, *p < 0.05 n.s.: not significant (p > 0.05); Tukey's HSD test. All cells were infected with lentivirus at an MOI of 5.

Back to article page