Table 1 Relative contents of nucleosides in C. sinensis, C. cicadae, C. militaris and C. gunnii were determined by LC-QTOF-MS/MS.

MEDN0136

1,7-Dimethylxanthine

[M−H]⁻

C₇H₈N₄O₂

9

0

9

0

1.23E+05

1.58E+04

9

0

MEDN0139

1-Methylxanthine

[M−H]⁻

C₆H₆N₄O₂

1.84E+05

1.14E+04

1.55E+06

6.72E+04

1.03E+05

1.40E+04

1.40E+05

1.25E+04

MEDN0140

Xanthine

[M−H]⁻

C₅H₄N₄O₂

6.40E+04

3.37E+04

5.78E+05

7.18E+04

3.01E+05

1.02E+05

1.09E+06

2.68E+05

MEDN0141

2′-Deoxyuridine

[M−H]⁻

C₉H₁₂N₂O₅

5.05E+04

5.83E+03

2.58E+05

1.19E+04

6.87E+05

4.54E+04

2.74E+05

2.50E+04

MEDN0149

7-Methylxanthine

[M−H]⁻

C₆H₆N₄O₂

3.80E+04

6.30E+03

3.04E+05

1.64E+04

2.50E+04

3.87E+03

4.04E+04

8.47E+03

MEDN0151

Adenine

[M−H]⁻

C₅H₅N₅

9.00E+00

0.00E+00

8.13E+04

4.14E+04

2.74E+05

5.95E+04

2.66E+05

2.97E+04

MEDN0153

Adenosine 5′-monophosphate

[M−H]⁻

C₁₀H₁₄N₅O₇P

5.83E+06

1.25E+06

1.31E+06

2.04E+05

3.43E+06

6.09E+05

2.63E+05

9.09E+04

MEDN0160

Guanosine

[M−H]⁻

C₁₀H₁₃N₅O₅

5.44E+07

3.43E+06

9.60E+07

9.70E+06

8.82E+07

4.11E+06

4.51E+07

1.40E+06

MEDN0165

Inosine 5′-monophosphate

[M−H]⁻

C₁₀H₁₃N₄O₈P

4.42E+05

9.55E+04

1.04E+05

1.95E+04

2.42E+05

2.03E+04

2.01E+04

5.48E+03

MEDN0166

Nicotinic acid adenine Dinucleotide

[M−H]⁻

C₂₁H₂₇N₇O₁₄P₂

3.64E+04

2.09E+04

1.55E+04

5.90E+03

1.10E+05

2.37E+04

1.88E+04

9.63E+03

MEDN0168

Thymidine

[M−H]-

C₁₀H₁₄N₂O₅

4.20E+04

7.04E+03

9.02E+04

1.63E+04

1.12E+05

4.73E+03

1.56E+05

8.06E+03

MEDN0169

Uracil

[M−H]⁻

C₄H₄N₂O₂

7.16E+04

8.61E+03

4.64E+04

8.74E+03

3.03E+05

4.53E+04

3.94E+04

1.84E+04

MEDN0171

Uridine

[M−H]⁻

C₉H₁₂N₂O₆

6.09E+04

1.72E+04

1.04E+05

2.34E+04

1.04E+05

2.20E+04

1.17E+05

1.49E+04

MEDN0306

Creatine phosphate

[M+H]⁺

C₄H₁₀N₃O₅P

1.06E+05

2.47E+04

7.88E+04

4.07E+03

6.42E+04

6.39E+03

2.02E+05

3.49E+04

MEDN0419

Uridine 5-monophosphate

[M−H]⁻

C₉H₁₃N₂O₉P

5.15E+06

9.07E+05

1.38E+06

2.25E+05

6.12E+05

2.34E+05

9

0

MEDN0428

N6-succinyl adenosine

[M−H]⁻

C₁₄H₁₇N₅O₈

2.46E+05

4.87E+03

3.03E+05

3.78E+04

1.21E+05

8.27E+03

2.33E+05

1.08E+04

MEDN0431

2-(Dimethylamino)guanosine

[M−H]⁻

C₁₂H₁₇N₅O₅

1.34E+04

1.01E+03

2.58E+06

8.66E+04

2.10E+06

5.73E+04

1.34E+05

4.11E+03

MEDN0433

8-Hydroxy-2-deoxyguanosine

[M−H]⁻

C₁₀H₁₃N₅O₅

1.22E+05

2.42E+04

6.40E+04

2.71E+03

4.27E+04

4.98E+03

5.95E+04

1.14E+04

MEDN0452

2′-Deoxycytidine-5′-monophosphate

[M−H]⁻

C₉H₁₄N₃O₇P

7.09E+04

2.02E+04

1.01E+05

1.86E+04

1.01E+05

1.38E+03

2.68E+05

9.46E+03

MEDN0533

Xanthosine

[M−H]⁻

C₁₀H₁₂N₄O₆

3.36E+05

3.50E+04

4.31E+06

6.45E+05

3.73E+06

3.09E+05

7.25E+06

2.12E+05

MEDN0537

ADP-ribose

[M−H]⁻

C₁₅H₂₃N₅O₁₄P₂

5.70E+04

8.33E+03

1.18E+04

3.12E+03

4.59E+04

2.79E+04

2.01E+04

1.46E+04

MEDN0538

UDP-glucose

[M−H]⁻

C₁₅H₂₄N₂O₁₇P₂

1.10E+05

2.57E+04

4.66E+04

8.69E+03

5.80E+06

9.84E+05

9

0

MEDN0540

1-Methylguanine

[M−H]⁻

C₆H₇N₅O

1.20E+04

2.64E+03

1.88E+05

1.72E+04

3.01E+04

2.71E+03

2.33E+04

3.60E+03

MEDN0568

2-Methylguanosine

[M−H]⁻

C₁₁H₁₅N₅O₅

3.76E+04

4.74E+03

1.05E+06

5.08E+04

8.88E+05

9.04E+04

1.08E+06

4.09E+04

MEDN0602

Deoxyguanosine 5′-monophosphate (dGMP)

[M−H]⁻

C₁₀H₁₄N₅O₇P

5.83E+06

1.25E+06

1.31E+06

2.04E+05

3.43E+06

6.09E+05

2.63E+05

9.09E+04

MEDN1003

Inosine

[M−H]⁻

C₁₀H₁₂N₄O₅

2.66E+07

2.00E+06

8.31E+07

7.02E+06

3.14E+07

1.25E+06

2.82E+07

4.75E+05

MEDN1012

7-Methylguanine

[M−H]⁻

C₆H₇N₅O

1.20E+04

2.64E+03

1.88E+05

1.72E+04

3.01E+04

2.71E+03

2.33E+04

3.60E+03

MEDN1013

2′-Deoxyadenosine-5′-monophosphate

[M−H]⁻

C₁₀H₁₄N₅O₆P

5.74E+04

8.20E+03

1.08E+05

1.04E+04

1.48E+05

2.83E+04

2.01E+05

1.83E+05

MEDN1045

2-Amino-4,6-pteridinediol

[M−H]⁻

C₆H₅N₅O₂

9

0

5.25E+04

1.80E+03

3.40E+05

6.75E+04

9

0

MEDN1076

N6-isopentene adenine

[M−H]⁻

C₁₀H₁₃N₅

9

0

4.44E+04

3.57E+03

7.68E+04

3.63E+03

9

0

MEDN1236

Cyclic ADP ribose

[M−H]⁻

C₁₅H₂₁N₅O₁₃P₂

4.62E+04

2.33E+04

1.61E+04

2.36E+03

8.45E+04

1.53E+04

2.38E+04

1.14E+04

MEDN1501

2′-O-methyluridine

[M−H]⁻

C₁₀H₁₄N₂O₆

8.86E+03

2.91E+03

5.32E+04

5.42E+03

6.88E+04

8.72E+03

4.09E+04

2.40E+03

MEDN1513

5-Hydroxy-2′-deoxyuridine

[M−H]⁻

C₉H₁₂N₂O₆

6.09E+04

1.72E+04

1.04E+05

2.34E+04

1.04E+05

2.20E+04

1.17E+05

1.49E+04

MEDN1515

5-Hydroxymethyl-2′-deoxycytidine

[M−H]⁻

C₁₀H₁₅N₃O₅

9

0

9

0

9

0

3.58E+05

6.47E+04

MEDN1525

Coproporphyrin III

[M−H]⁻

C₃₆H₃₈N₄O₈

9

0

2.06E+04

5.91E+03

3.12E+04

2.55E+03

1.52E+04

3.22E+03

MEDN1529

Cytidine 5′-monophosphate-N-acetylneuraminic acid (CMP-NANA)

[M−H]⁻

C₂₀H₃₁N₄O₁₆P

1.22E+04

7.59E+03

5.32E+03

2.62E+03

4.75E+03

1.96E+03

1.45E+04

8.47E+03

MEDN1586

Thymidine-5′-phosphate (dTMP)

[M−H]⁻

C₁₀H₁₅N₂O₈P

1.65E+05

3.83E+04

6.07E+05

4.13E+04

3.84E+05

6.48E+04

5.29E+05

4.86E+05

MEDN1602

3-Deoxyguanosine

[M−H]⁻

C₁₀H₁₃N₅O₄

1.88E+06

4.43E+04

5.00E+06

4.09E+05

1.26E+07

4.84E+05

4.24E+05

4.82E+04

MEDN1673

6-Benzylaminopurine

[M−H]⁻

C₁₂H₁₁N₅

9

0

9

0

1.32E+04

2.39E+03

1.40E+03

2.40E+03

MEDN1675

2-Deoxyribose-5′-phosphate

[M−H]⁻

C₅H₁₁O₇P

7.55E+04

1.37E+04

5.43E+04

8.83E+03

7.97E+04

2.23E+04

5.13E+04

1.84E+04

MEDN1677

Adenosine 2′-phosphate

[M−H]⁻

C₁₀H₁₄N₅O₇P

7.06E+06

5.14E+05

4.56E+06

5.73E+05

1.79E+06

2.15E+05

1.50E+06

2.66E+05

MEDP0146

1-Methyladenine

[M+H]⁺

C₆H₇N₅

5.63E+04

3.89E+04

9.63E+04

2.60E+04

6.90E+05

8.01E+04

3.54E+05

2.59E+04

MEDP0150

2′-Deoxyinosine

[M+H]⁺

C₁₀H₁₂N₄O₄

1.59E+05

2.09E+04

1.31E+06

3.45E+04

8.35E+07

2.14E+06

1.11E+06

2.99E+04

MEDP0151

2-Hydroxy-6-aminopurine

[M+H]⁺

C₅H₅N₅O

2.19E+06

5.93E+04

9.77E+06

1.06E+06

2.97E+06

4.78E+05

5.15E+06

8.22E+05

MEDP0152

3′-Aenylic acid

[M+H]⁺

C₁₀H₁₄N₅O₇P

2.13E+08

3.75E+07

1.60E+07

1.77E+06

5.72E+07

1.48E+06

5.99E+06

3.77E+05

MEDP0153

3-Methylxanthine

[M+H]⁺

C₆H₆N₄O₂

4.23E+06

2.58E+05

9

0

8.67E+04

5.89E+03

9

0

MEDP0155

5-Methylcytosine

[M+H]⁺

C₅H₇N₃O

8.99E+04

1.54E+04

2.19E+06

1.63E+05

6.57E+05

4.73E+04

2.27E+05

4.51E+04

MEDP0156

5-Methyluridine

[M+H]⁺

C₁₀H₁₄N₂O₆

1.92E+05

3.54E+04

5.18E+05

5.62E+04

1.97E+05

2.26E+04

3.28E+05

5.24E+04

MEDP0160

Adenosine

[M+H]⁺

C₁₀H₁₃N₅O₄

5.95E+08

1.76E+07

5.79E+08

2.33E+07

4.85E+08

1.68E+07

5.91E+08

1.71E+07

MEDP0163

Cytidine

[M+H]⁺

C₉H₁₃N₃O₅

6.56E+06

1.40E+06

5.16E+06

5.79E+05

7.13E+06

7.94E+05

1.50E+07

8.27E+05

MEDP0164

Cytidine-5-monophosphate

[M+H]⁺

C₉H₁₄N₃O₈P

2.79E+06

5.16E+05

5.26E+05

1.78E+05

1.02E+06

3.86E+05

7.86E+05

2.22E+05

MEDP0166

Deoxyguanosine

[M+H]⁺

C₁₀H₁₃N₅O₄

5.95E+08

1.76E+07

5.79E+08

2.33E+07

4.85E+08

1.68E+07

5.91E+08

1.71E+07

MEDP0168

Guanosine 3′,5′-cyclic monophosphate

[M+H]⁺

C₁₀H₁₂N₅O₇P

1.90E+05

8.07E+03

1.93E+06

6.61E+04

6.97E+05

5.16E+04

1.62E+06

6.33E+05

MEDP0169

Guanosine-5′-monophosphate

[M+H]⁺

C₁₀H₁₄N₅O₈P

7.72E+06

1.88E+06

2.50E+06

1.90E+05

1.60E+06

1.15E+05

1.64E+06

2.60E+05

MEDP0170

Hypoxanthine

[M+H]⁺

C₅H₄N₄O

2.80E+06

4.04E+05

2.40E+06

5.22E+05

1.42E+06

1.04E+05

8.39E+06

9.44E+05

MEDP0177

Thymine

[M+H]⁺

C₅H₆N₂O₂

5.60E+05

2.31E+04

5.83E+05

2.77E+04

1.75E+06

2.05E+05

1.07E+06

6.06E+04

MEDP0180

Β-Nicotinamide mononucleotide

[M+H]⁺

C₁₁H₁₅N₂O₈P

4.04E+04

1.20E+04

9

0

2.24E+05

4.94E+04

9

0

MEDP0374

Cyclic Amp

[M+H]⁺

C₁₀H₁₂N₅O₆P

1.81E+06

3.39E+04

1.09E+06

5.73E+04

1.15E+07

4.60E+05

1.06E+06

3.85E+04

MEDP0383

Β-Pseudouridine

[M+H]⁺

C₉H₁₂N₂O₆

1.08E+06

9.66E+04

1.12E+06

5.50E+04

1.23E+06

2.15E+05

9.32E+05

6.88E+04

MEDP0390

Hypoxanthine-9-β-d-arabinofuranoside

[M+H]⁺

C₁₀H₁₂N₄O₅

2.48E+08

1.31E+07

2.03E+08

4.83E+06

1.29E+08

5.55E+06

1.95E+08

7.98E+06

MEDP0401

5′-Deoxy-5′-(methylthio) adenosine

[M+H]⁺

C₁₁H₁₅N₅O₃S

6.52E+07

3.59E+06

6.35E+06

9.74E+05

3.69E+08

9.95E+06

5.27E+06

5.96E+05

MEDP0403

Deoxycytidine

[M+H]⁺

C₉H₁₃N₃O₄

7.95E+04

5.63E+03

3.15E+05

2.39E+04

3.90E+05

5.68E+04

4.28E+05

6.76E+04

MEDP0526

6-Dimethylaminopurine

[M+H]⁺

C₇H₉N₅

1.69E+06

1.67E+05

4.06E+06

5.36E+05

1.09E+06

5.74E+04

2.18E+07

8.70E+05

MEDP0881

Phosphocholine

[M+H]⁺

C₅H₁₅NO₄P

2.70E+07

4.10E+06

2.54E+07

2.72E+06

3.05E+07

1.07E+06

2.05E+07

9.83E+04

MEDP0899

3-Methyladenine

[M+H]⁺

C₆H₇N₅

5.63E+04

3.89E+04

9.63E+04

2.60E+04

6.90E+05

8.01E+04

3.54E+05

2.59E+04

MEDP1010

Coenzyme II (β-NADP)

[M+H]⁺

C₂₁H₂₈N₇O₁₇P₃

9

0

6.64E+04

8.82E+03

2.14E+04

9.00E+03

9

0

MEDP1018

5′-Deoxyadenosine

[M+H]⁺

C₁₀H₁₃N₅O₃

4.09E+05

2.38E+04

3.72E+06

5.86E+04

2.54E+08

4.73E+05

3.14E+06

7.92E+04

MEDP1072

7-Methylguanosine

[M+H]⁺

C₁₁H₁₅N₅O₅

2.53E+05

2.03E+04

5.41E+06

3.27E+05

3.76E+06

1.42E+05

8.82E+06

1.31E+05

MEDP1073

6-O-methylguanine

[M+H]⁺

C₆H₇N₅O

7.39E+05

1.60E+05

1.92E+07

9.05E+05

8.41E+05

1.83E+05

1.96E+06

8.30E+04

MEDP1077

2′-O-methyladenosine

[M+H]⁺

C₁₁H₁₅N₅O₄

1.84E+08

3.82E+07

1.31E+07

1.34E+06

5.43E+07

1.29E+06

5.28E+06

1.23E+06

MEDP1083

Oxypurinol

[M+H]⁺

C₅H₄N₄O₂

1.42E+07

6.07E+05

3.21E+07

1.71E+06

2.47E+07

3.31E+05

3.56E+07

2.89E+06

MEDP1093

Allopurinol

[M+H]⁺

C₅H₄N₄O

9

0

1.95E+07

1.09E+06

6.70E+06

4.55E+05

1.45E+07

9.22E+05

MEDP1100

2-Aminomethylpyrimidine

[M+H]⁺

C₅H₇N₃

1.46E+07

1.49E+06

1.00E+07

1.13E+06

1.55E+07

2.02E+05

1.39E+06

1.06E+05

MEDP1193

Flavin single nucleotide (FMN)

[M+H]⁺

C₁₇H₂₁N₄O₉P

5.39E+06

5.00E+05

2.44E+06

1.02E+05

1.58E+06

1.34E+05

1.88E+06

1.50E+05

MEDP1202

5′-Deoxy-5′-fluoroadenosine

[M+H]⁺

C₁₀H₁₂FN₅O₃

3.90E+07

2.01E+06

3.17E+07

1.44E+06

2.14E+07

9.14E+05

3.03E+07

1.00E+06

MEDP1276

Guanine

[M+H]⁺

C₅H₅N₅O

2.19E+06

5.93E+04

9.77E+06

1.06E+06

2.97E+06

4.78E+05

5.15E+06

8.22E+05

MEDP1295

Cytarabine

[M+H]⁺

C₉H₁₃N₃O₅

1.44E+06

1.76E+05

2.34E+06

1.89E+05

2.47E+06

1.92E+05

5.05E+06

6.97E+05

MEDP1493

N6-methyladenosine

[M+H]⁺

C₁₁H₁₅N₅O₄

7.30E+05

6.74E+04

8.53E+06

1.42E+05

1.23E+06

4.02E+04

2.22E+07

1.43E+06

MEDP1498

8-Azaguanine

[M+H]⁺

C₄H₄N₆O

9.44E+06

5.57E+05

2.21E+07

4.92E+05

1.64E+07

8.26E+05

2.49E+07

1.87E+06

MEDP1501

Isocytosine

[M+H]⁺

C₄H₅N₃O

1.60E+06

9.18E+04

1.50E+06

1.82E+05

2.07E+06

5.34E+05

2.94E+06

3.46E+05

MEDP1654

1-Methyladenosine

[M+H]⁺

C₁₁H₁₅N₅O₄

7.30E+05

6.74E+04

8.53E+06

1.42E+05

1.23E+06

4.02E+04

2.22E+07

1.43E+06

MEDP1712

1-Methylguanosine

[M+H]⁺

C₁₁H₁₅N₅O₅

2.75E+05

1.08E+04

5.98E+06

4.08E+05

3.96E+06

3.07E+04

8.95E+06

1.72E+05

MEDP1713

1-Methylinosine

[M+H]⁺

C₁₁H₁₄N₄O₅

9

0

5.33E+05

1.00E+05

1.91E+05

1.48E+04

2.88E+05

5.39E+04

MEDP1714

2′-Deoxyadenosine

[M+H]⁺

C₁₀H₁₅N₅O₄

1.06E+05

3.21E+04

2.09E+06

3.63E+05

2.84E+05

4.88E+04

2.90E+05

4.97E+04

MEDP1717

2′-O-methylcytidine

[M+H]⁺

C₁₀H₁₅N₃O₅

1.06E+06

1.14E+05

2.94E+06

1.13E+05

3.56E+07

2.40E+06

1.44E+06

2.21E+05

MEDP1719

2′-O-methylguanosine

[M+H]⁺

C₁₁H₁₅N₅O₅

2.75E+05

1.08E+04

5.98E+06

4.08E+05

3.96E+06

3.07E+04

8.95E+06

1.72E+05

MEDP1736

5-Methylcytidine

[M+H]⁺

C₁₀H₁₅N₃O₅

1.06E+05

1.57E+04

7.53E+06

2.53E+06

3.57E+04

6.65E+03

2.11E+05

3.41E+04

MEDP1787

N4-Acetylcytidine

[M+H]⁺

C₁₁H₁₅N₃O₆

4.50E+05

6.90E+04

1.35E+06

3.31E+04

2.04E+06

3.38E+04

6.57E+05

1.04E+05

MEDP1788

N4-Acetylcytidine triphosphate

[M+H]⁺

C₁₁H₁₈N₃O₁₅P₃

1.21E+07

3.84E+05

4.12E+05

8.99E+04

1.21E+07

7.63E+05

1.07E+07

5.67E+05

MEDP1791

Nicotinamide riboside (chloride)

[M-Cl]⁺

C₁₁H₁₅ClN₂O₅

5.97E+04

1.22E+04

1.43E+05

2.17E+04

5.42E+05

7.44E+04

9.00E+00

0.00E+00

MEDP1949

5-Aminoimidazole ribonucleotide

[M+H]⁺

C₈H₁₄N₃O₇P

1.73E+07

3.34E+06

5.13E+06

3.33E+05

1.35E+07

2.58E+06

3.03E+06

2.92E+05

MEDP1950

2′-Deoxyinosine-5′-monophosphate

[M+H]⁺

C₁₀H₁₃N₄O₇P

1.65E+07

2.69E+06

2.42E+05

7.46E+04

1.65E+05

8.50E+03

1.06E+07

9.36E+05

MEDP1954

N6-(2-Hydroxyethyl)adenosine

[M+H]⁺

C₁₂H₁₇N₅O₅

1.39E+05

2.94E+04

2.35E+08

1.20E+07

2.18E+08

9.01E+06

1.58E+06

8.41E+04

MEDP1955

3′-Deoxyadenosine

[M+H]⁺

C₁₀H₁₃N₅O₃

4.09E+05

2.38E+04

3.72E+06

5.86E+04

2.54E+08

4.73E+05

3.14E+06

7.92E+04

MEDP1956

2-Aminopurine

[M+H]⁺

C₅H₅N₅

6.13E+06

2.76E+05

4.93E+06

3.16E+05

2.43E+06

1.79E+05

4.79E+06

2.31E+05

MEDP1959

N6-(4-hydroxybenzyl)adnine riboside

[M+H]⁺

C₁₂H₁₁N₅O

9

0

3.07E+05

9.23E+04

1.41E+06

2.05E+05

9

0

MEDP1960

Isopentenyladenine-7-N-glucoside

[M+H]⁺

C₁₆H₂₃N₅O₅

1.13E+06

4.08E+05

1.32E+06

2.34E+05

2.18E+06

2.66E+05

9.42E+05

1.17E+05

MEDP1961

Ribosyladenosine

[M+H]⁺

C₁₅H₂₁N₅O₈

5.90E+05

8.22E+04

2.39E+06

1.17E+05

9.02E+06

7.88E+05

1.94E+07

1.09E+06

MEDP2049

N-Benzoyl-2′-deoxycytidine

[M+H]⁺

C₁₆H₁₇N₃O₅

1.56E+05

8.90E+03

1.54E+05

1.44E+04

1.81E+05

4.25E+04

1.84E+05

5.96E+03

MEDP2053

Xanthosine-5′-monophosphate

[M+H]⁺

C₁₀H₁₃N₄O₉P

7.12E+05

2.15E+05

2.46E+05

3.39E+04

1.46E+05

5.87E+04

1.14E+05

2.06E+04