Figure 2 | Scientific Reports

Figure 2

From: Improvement of the cell viability of hepatocytes cultured in three-dimensional collagen gels using pump-free perfusion driven by water level difference

Figure 2

Continuous flow of fresh medium is important for the maintenance of cell viability. (a) Fluorescent images after 3 days of culture with PCC-WLD based on the live/dead assay. The medium was replaced every 24 h. Scale bar, 200 μm. (b) Analysis of the live/dead assay between 12 and 24 h medium replacement. (c,d) Fluorescent images (c) and analysis (d) of the perfusion culture with fresh or supernatant medium based on live/dead cell assay. The water level difference was restored every 12 h for 3 days. (a,c) Cyan, Hoechst. Green, phalloidin. Magenta, RIF. Scale bar, 200 μm. (b,d) n = 9 fields from 3 independent experiments. Mean ± S.E. *p < 0.0001 by Welch’s t test.

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