Figure 4 | Scientific Reports

Figure 4

From: Improvement of the cell viability of hepatocytes cultured in three-dimensional collagen gels using pump-free perfusion driven by water level difference

Figure 4

Gel volume, but not collagen concentration, affected cell viability. (a, b) Fluorescent images (a) and analysis (b) of cells embedded in 200 μL of gel with different concentrations of collagen based on the live/dead cell assay. (c,d) Fluorescent images (c) and analysis (d) of cells embedded in 2.4 mg/mL collagen gels with different volumes based on the live/dead cell assay. Cells were cultured for 3 days with PCC-WLD with a syringe. The center of the gel was observed. Cyan, Hoechst. Green, phalloidin. Magenta, RIF. Scale bar, 200 μm. n = 3 fields from 3 independent experiments. Mean ± S.E. n.s. no significance. *p < 0.05 by Student’s t-test with Bonferroni correction.

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