Figure 3

Low protein fed mice illustrate changed mitochondrial morphology and markers of function which were mitigated by rapamycin. (a) TEM magnification × 4000, scale bar 2um, red square encircles a mitochondria with an inclusion body, # indicate autophagosomes. (b) HSP60 IF staining in red, scale bar 10um. (c) mitochondrial DNA. (d) TOM20 quantification of western blot relative to 18% diet. (e) Westernblots. (f) Relative expression Tfam. (g) Relative expression NRF1. (h) Complex I quantification of western blot relative to 18% diet. (i) Hepatic ATP levels, nmol/g wet weight. (j) PINK1 quantification of western blot relative to 18% diet. The westerns were cut prior to incubation with the primary antibodies, the full size blots can be seen in Supplementary Fig. S7. *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001.