Figure 4
From: Inhibition of mTOR improves malnutrition induced hepatic metabolic dysfunction
Rapamycin treatment significantly increased autophagy markers. (a). Western blots LC3, p62, GAPDH. Western blot quantification of and shown as a relative expression to 18% control diet (b) total LC3-II/GAPDH. (c) LC3-II to I ratio. (d) p62/GAPDH. (e) Western blots S6K, GAPDH, phos-S6K, beta-actin. Western blot quantification of and shown as a relative expression to 18% control diet (f) S6K/GAPDH. (g) phos-S6K. (h) phos-S6K/S6K ratio. (i) Western blots ULK1, GAPDH, phos-ULK1 (j) ULK1/GAPDH. (k) phos-ULK1/GAPDH. (l) phos-ULK1:ULK ratio. The westerns were cut prior to incubation with the primary antibodies, the full size blots can be seen in Supplementary Fig. S9. *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001.
