Figure 5
From: Inhibition of mTOR improves malnutrition induced hepatic metabolic dysfunction
A low protein diet impairs the TCA cycle, which is in part prevented by rapamycin treatment. (a) Left panel, score plot of PLS-DA showing group separation based on all measured metabolites (groups coded as per legend); Right panel, correlation plot presenting the relationships between the top distinctive metabolites (VIP > 1); the metabolites that significantly contribute to component-1 (i.e., control vs. low protein fed) are indicated with black lines, those in grey show metabolites that only contribute to component-2 (i.e., difference between rapamycin treated vs. untreated low protein fed animals); while metabolites that contribute to both component-1 and -2 are indicated by orange lines. (b) Bar plots presenting mean and SEM of selected top metabolites that most discriminate between groups, nmol/g. n = 5. (c) Schematic overview of the changes in metabolites seen in the low protein fed-mice, red arrow indicating the increase or decrease in metabolites compared to control mice, and blue arrow compared to rapamycin. *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001.
