Figure 3

Cerebral mitochondrial respiration. (a) Maximal oxidative phosphorylation (OXPHOS) system capacity and (b) electron transport system (ETS) capacity supported by mitochondrial complex I and II (CI + II)-linked substrates, as well as (c) complex CIV(CIV)-linked mitochondrial respiration supported by the artificial electron donor N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride were measured in brain homogenates of sham animals (Sham), untreated animals exposed to diisopropylfluorophosphate alone (DFP), animals exposed to DFP receiving standard of care (SOC) treatment (DFP + SOC), animals exposed to DFP receiving SOC treatment and the cell-permeable succinate prodrug NV354 (DFP + SOC + NV354) and animals exposed to DFP receiving the cell-permeable succinate prodrug NV354 (DFP + NV354). Data are presented as median plus interquartile range, with whiskers indicating minimal and maximal values. Kruskal–Wallis test or One-Way ANOVA was applied for analysis of differences of non-normally distributed and normally distributed data, respectively, with Tukey’s multiple comparison of every group to every other group. *p < 0.05 and **p < 0.01 indicate significant difference between groups. n = 6 per group.