Figure 1
Shoot organogenesis from leaf explants in Euryodendron excelsum. (a) Leaf explants derived from axillary shoots on PGR-free WPM for 4 weeks. (b) Callus was induced on WPM with 1.0 mg/L 2,4-D after culture for 6 weeks. (c) Callus and adventitious roots were induced on WPM with 1.0 mg/L NAA after culture for 6 weeks. (d) Adventitious shoots were induced on WPM with 1.0 mg/L TDZ and 0.2 mg/L NAA after culture for 6 weeks. (e) Callus induced on WPM with 1.0 mg/L 2,4-D cultured for 6 weeks, then they were transferred to on WPM with 1.0 mg/L BA and 0.2 mg/L NAA for an additional 3 weeks, the callus differentiated into adventitious shoots. (f) Callus induced on WPM with 1.0 mg/L 2,4-D cultured for 6 weeks, then differentiated into adventitious shoots (white arrow) on PGR-free WPM after 21 d. (g) Callus induced on WPM with 1.0 mg/L 2,4-D cultured for 6 weeks, then differentiated into globular and torpedo-shaped somatic embryos (white arrows) on PGR-free WPM for 4 weeks. (h) Callus induced on WPM with 1.0 mg/L 2,4-D after culture for 6 weeks, then differentiated into adventitious shoots (red arrow) and somatic embryos (white arrows) on PGR-free ½WPM for 4 weeks. (i) Callus induced on WPM with 1.0 mg/L 2,4-D after culture for 6 weeks, then differentiated into cotyledon-shaped somatic embryo-like structure that developed roots on PGR-free WPM after 4 weeks. Bars = 1.0 cm.
