Table 2 Differentiation into shoots and somatic embryos of callus induced by 1.0 mg/L 2,4-D for 6 weeks and then transferred to the different media for differentiation in Euryodendron excelsum.

From: Shoot organogenesis and somatic embryogenesis from leaf and petiole explants of endangered Euryodendron excelsum

PGRs in WPM (mg/L)

Morphogenesis

Number of shoots/callus clump

Number of somatic embryos/callus clump

PGR-free

Shoots and somatic embryos

1.8 ± 0.1 b

2.5 ± 0.2 c

NAA 1.0

Somatic embryos

0 c

3.7 ± 0.2 b

Zeatin 1.0

Somatic embryos

0 c

6.4 ± 0.4 a

TDZ 1.0

Adventitious shoots

3.4 ± 0.7 a

0 d

BA 1.0

Adventitious shoots

3.3 ± 0.7 a

0 d

KIN 1.0

Adventitious shoots

3.1 ± 0.6 a

0 d

  1. *Each treatment had 30 callus clumps. Values represent means ± SD. Different letters within a column indicate significant differences according to Duncan’s multiple range test (P ≤ 0.05).
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