Figure 5 | Scientific Reports

Figure 5

From: Transcriptomic comparison of primary human lung cells with lung tissue samples and the human A549 lung cell line highlights cell type specific responses during infections with influenza A virus

Figure 5

Viral sequences retrieved from infected samples show comparable infection rates across all cell types. Sequences obtained from RNA sequencing were mapped against a hybrid reference genome of human and H3N2 Panama virus. (A) Transcripts per million (TPM) and cell type are shown as mean + SEM. Uninfected cells (not shown) showed TPMs below 10 for viral transcripts. (B) Genes from the shared upregulated pool (302 genes) that correlate (Pearson > 0.7) with the ratio of viral NA mRNA to cellular β-actin (ACTB) mRNA (n = 29) show a mean similar induction across all cell types (B). These genes show a high enrichment of the cellular defence response pathways (C). While the relative induction is similar, abundance across all cell types is different as shown by z-score (D). Classifications: BP = biological process, CC = cell compartment, MP = molecular process, KEGG = Kyoto Encyclopedia of Genes and Genomes, REAC = reactome database.

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