Figure 3

Overview of methylation changes of individual metastases pairs. Patient-specific summary of the HMM-based predictions of the methylation states of individual CpGs. The patient-specific metastases pairs are sorted by the tissue in which the extracranial metastasis occurred (x-axis) with brain versus lung (blue), brain versus lymph node (green), brain versus skin (yellow), brain versus soft tissue (purple), and brain versus liver (pink). (A) Total number of differentially methylated CpGs predicted to have a decreased (blue, downward bars from 0) or increased (red, upward bars from 0) methylation in the corresponding patient-specific metastases pair. (B) Percentage of CpGs with decreased or increased methylation located in functional genomic elements with a focus on gene bodies, promoters and enhancers for each metastases pair. (C) Percentage of increased and decreased methylated CpGs of the most frequently affected signaling pathways. Significant enrichment of a genomic category is marked with a red ‘x’ in subpanels (B) and (C) (FDR-adjusted p value < 0.05).