Figure 2
Heterogeneous tissue regeneration by GS application occurs in deep wounds with periosteal defects in a BMT rat model. (a) Schema of establishing BMT rats. After 9 Gy irradiation exposure, 1 × 108 bone marrow cells isolated from green fluorescent protein (GFP) rats were injected into the tail vein of recipient rats. One month later, skin/subcutaneous tissue/periosteum defects were made in the calvaria with/without GS application. n = 4 for each. (b) Gross appearance of the deep wounds with or without GS application is shown at various time points. (c) Wound area with or without GS application was quantified. (d–t) H and E staining of the wounds with or without GS application are shown. The experiments were repeated twice. Scale bars, 5 mm in c, 1 mm in (d,g,j,m,p,t) 100 μm in (e,f,h,i,k,l,n,o,r,u,v). Arrowheads, see the text. B bone cortex. *P < 0.05, **P < 0.01, ***P < 0.005.
