Figure 5
Quantitative analyses in regenerating tissue due to GS application. (a) Schema of the tissues in the rat calvaria. The dermis is defined as the tissue from the epidermis–dermis junction to a depth of 400 μm, and from there to the periosteum, it is further defined as subcutaneous tissue. The green areas of either squares or rectangles were analysed. (b,c) The changes in the rates of GFP migration into the periosteum and the periosteal thickness in control (unwounded healthy BMT rats), GS (−), and GS (+) rats at 4 w and 6 w pw. (d,e) The changes in the rates of GFP migration into subcutaneous tissues and the numbers of vessels in control, GS (−), and GS (+) rats at 4 w and 6 w pw. (f,g) Changes in the rates of GFP migration into the dermis and the numbers of vessels in control, GS (−), and GS (+) rats at 4 w and 6 w pw. The cross of each scatter or box plot indicates average value. The upper or lower whisker of each box plot indicates maximal or minimal value, respectively. n = 4 for each. The experiments were repeated twice. P < 0.05, **P < 0.01, ***P < 0.005.
