Figure 8 | Scientific Reports

Figure 8

From: Combination of esomeprazole and pirfenidone enhances antifibrotic efficacy in vitro and in a mouse model of TGFβ-induced lung fibrosis

Figure 8

Measurement of collagen in lung homogenates from TGFβ-induced lung fibrosis mouse model. The right lungs of vehicle, esomeprazole, pirfenidone, and esomeprazole/pirfenidone combination were homogenized and analyzed for tissue collagen content by colorimetric assay. The homogenates from the right lungs were pooled together for each group (n = 5 animals/group) and assayed using Sircol assay following the manufacturer’s protocol. The amount of collagen was estimated from standard curves (OD = 555 nm) and was expressed as µg collagen per mg of wet tissue. Data is mean ± SEM from triplicate experiments. *p < 0.05 compared to TGFβ only control and +p < 0.05 vs pirfenidone alone group.

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