Figure 1
From: SSTR2 as an anatomical imaging marker and a safety switch to monitor and manage CAR T cell toxicity
PEN-221 induces internalization of SSTR2 and exhibits specific cytotoxicity against SSTR2-expressing Jurkat cells in vitro. (a) Schematic representation of the lentiviral vector encoding human SSTR2 (SSTR2). Histograms show the expression of SSTR2 in non-transduced (NT) Jurkat and Jurkat-SSTR2 cells determined by flow cytometry. (b) Representative flow-cytometric measurements of SSTR2 internalization in Jurkat-SSTR2 cells after treatment with 10-folds serially diluted PEN-221 (10–6 to 10–12 M). (c) EC50 curve of SSTR2 internalization. The mean fluorescence intensity of surface SSTR2 is indicative of the internalization of the receptor. (d) Cytotoxicity of PEN-221 against SSTR2-expressing Jurkat T cells, using Jurkat-NT cells as control. Data were presented as the mean ± SD of triplicate wells.
