Figure 4

Mortality curves of T. molitor after cold stress (COLD, light blue line), injection of Tenmo-MIP 5 neuropeptide at a concentration of 10–6 M, giving a final concentration in the haemolymph of 10–7 M (MIP, green line), injection of neuropeptide and cold stress (COLD + MIP, dark blue line), injection of physiological saline (PS, brown line) and control (black line). Values are given as the mean ± SEM (n = 3, Note that because not all animals survived all the way through this experiment, some experimental groups only have one survivor after 21 days). The differences between survival curves were estimated based on the log-rank (Mantel‒Cox) test, *p ≤ 0.05; **p ≤ 0.01.