Figure 5

Quantitative analysis of the expression levels of immune-related genes in the fat body of T. molitor after cold stress (COLD), injection of Tenmo-MIP 5 at a concentration of 10–6 M, giving a final concentration in the haemolymph of 10–7 M (MIP), combined cold stress and neuropeptide injection (COLD + MIP) and physiological saline injection (PS). Values are given as the mean ± SEM (n = 3 biological × 2 technical replications). Different letters show statistically significant differences among treatments within each group at p ≤ 0.05 (one-way ANOVA).