Figure 4
From: Overexpression of hepatocyte EphA2 enhances liver-stage infection by Plasmodium vivax
EphA2Extra-HC04 cells enhanced P. vivax liver-stage infection. (A) Fold increase of P. vivax infection of the two EphA2Extra-HC04 clones relative to the original HC-04. Error bars represent S.E.M. of 8 biological replicates. The p-value for two-tail paired t-test between EphA2Extra-HC04 cell clones and original HC-04. (B) P. vivax-infected EphA2Extra-HC04 (top) and original HC-04 (bottom) were labeled with a HA-tag antibody (green). Liver-stage parasites were labeled with a UIS4 antibody (red). Nuclei were stained with DAPI (blue). The scale bar represents 5 μm. (C) The size distribution of liver-stage parasites in each cell line. The diameters of intrahepatic parasites on day 4 post-infection were pooled from eight P. vivax clinical isolates. Dashed lines represent the medians; dotted lines delimit the interquartile ranges. ****Indicates a p-value < 0.0001 and ns indicates a non-significant test result (p = 0.0761) by the Mann–Whitney test between the indicated pair.
