Figure 2 | Scientific Reports

Figure 2

From: Novel expression cassettes for increasing apolipoprotein AI transgene expression in vascular endothelial cells

Figure 2

Addition of Mef2c enhancers to APOAI expression cassettes. (a) Bovine aortic endothelial cells (BAEC) were transfected with pBshuttle-4XETE-gApoAI-oPRE containing 0–5 copies of a Mef2c enhancer. (b) BAEC were transfected with pBshuttle-1XETE-gApoAI-oPRE containing 0–5 copies of a Mef2c enhancer, or with pBshuttle-4XETE-gApoAI-oPRE. (a,b) APOAI mRNA was measured by RT-qPCR, 24 h after transfection, and is expressed in arbitrary units (AU). (b) Median APOAI expression for 1XETE-gApoAI-oPRE with 0 Mef2c copies is assigned a value of 1; all other points are expressed relative to this value. (a,b) Data are from three independent experiments; bars indicate group medians. (a) **P < 0.01; ***P < 0.001 vs. 4XETE-gApoAI-oPRE with 0 Mef2c copies. (b) *P < 0.05; ***P < 0.001 vs. 1XETE-gApoAI-oPRE with 0 Mef2c copies.

Back to article page