Figure 1
Immunogenicity of S-910823 plus A-910823 in mice after intramuscular dosing. (a) Study schedule schematic. (b) Antigen-specific total IgG, IgG1, and IgG2a titers in the serum were measured using enzyme-linked immunosorbent assays. Each bar represents the geometric mean titer (error bars indicate a 95% confidence interval). Each circle represents the titer in an individual mouse. Dotted lines indicate the lower limit of detection. (c) Serum titers of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Each bar represents the geometric mean titer (error bars indicate a 95% confidence interval). Each circle represents the neutralizing antibody titer in individual mice. The dotted line indicates the lower limit of detection. (d) Serum titers of neutralizing antibodies against SARS-CoV-2 spike pseudotyped lentivirus in mice administered 1 μg of S-268019-b. Each bar represents the geometric mean titer (error bars indicate a 95% confidence interval). Each circle represents the neutralizing antibody titer in individual mice. The dotted line indicates the lower limit of detection. (e) The number of cytokine-secreting cells in the spleen of immunized mice was determined using enzyme-linked immunospot analysis. Each bar represents mean spot-forming cells (SFCs) (error bars indicate standard error of mean). The open and solid bars indicate non-stimulated splenocytes and splenocytes stimulated with SARS-CoV-2 spike-protein peptides, respectively. The gray bars represent mice administered S-910823 without A-910823, while the rose bars represent mice administered S-268019-b. The open and solid circles represent the results of individual mice.
