Figure 4
Effect of A-910823 on S-910823 immunogenicity. (a) Study schedule schematic. (b) Physical association of S-910823 with A-910823 in mice. S-910823 (1 μg) and A-910823 (100% v/v) were separately administered into mice at different injection intervals (0 h, 1 h, and 24 h) on Day 0 and Day 14 (n = 10/group). Serum titers of neutralizing antibodies against authentic SARS-CoV-2 virus were measured in mice at 2 wk post-second immunization. Each bar represents the geometric mean titer (error bars indicate a 95% confidence interval). The circles represent the results of individual animals. The dashed line indicates the lower limit of detection. Statistical significance was determined using one-way analysis of variance, followed by Tukey’s multiple comparison test (*P < 0.05, ***P < 0.001, and ****P < 0.0001). (c) Cytokine and chemokine concentrations in the muscle homogenate supernatant at 3 h, 6 h, 24 h, and 72 h post-administration of 50% (v/v) A-910823 or vehicle (n = 4–7 per time point). Data are represented as mean ± standard error. (d) Quantification of inflammatory cells in the draining lymph nodes at 3 h and 24 h post-administration of vehicle or 1 μg of S-910823 with or without A-910823 (n = 6/group). The bars represent the mean number of cells (error bars indicate standard error of mean). The circles represent the results of individual animals.
