Figure 1 | Scientific Reports

Figure 1

From: MDMX elevation by a novel Mdmx–p53 interaction inhibitor mitigates neuronal damage after ischemic stroke

Figure 1

Mdmx exerts a neuroprotective role in hypoxia/recovery. (A–C) Western blot (A) and quantification of Mdmx (B) and p-Mdmx (C) in mice contralateral and penumbra. (D–F) Western blot (D) and quantification of Mdmx (E) and p-Mdmx (F) in neurons, before and after hypoxia/recovery. N = 3. (G) Successful cell transduction is demonstrated by expression of AAV-GFP. Scale Bar = 100 μm. (H,I) Western blot (H) and quantification of Mdmx (I) in AAV-GFP, AAV-Mdmx and AAV-Mdm2 transduced neurons. N = 3. (J,K) Immunofluorescence Stanning (J) and quantification (K) of Mdmx/MAP2 in AAV-GFP and AAV-Mdmx transduced neurons. N = 6. Scale Bar = 100 μm. (L,M) Immunofluorescence Stanning (L) and quantification (M) of Mdmx/MAP2 in AAV-Mdmx transduced neurons, before and after OGD. N = 6. Scale Bar = 100 μm. (N) Cell Viability Assay of AAV-GFP and AAV-Mdmx transduced neurons before and after hypoxia/recovery. N = 4.

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