Figure 4
K-181 increases Mdmx expression under ischemic stroke in mice brains. (A–I) Western Blot images (A) and quantification of Mdmx (B), p53 (C), Mdm2 (D), p21 (E), E2F1 (F), cleaved Caspase-3 (G), TNF-α (H) and p53 (acetyl Lys379) (I) expression in vehicle and K-181 treated mice brains. (J–K) Representative immunostaining images (J) and quantification of p53 (K) in vehicle and K-181 treated mice brains. N = 6. Scale Bar = 100 μm. (L–M) Representative immunostaining images (L) and quantification of Caspase-3 (M) in vehicle and K-181 treated mice brains. N = 6. Scale Bar = 100 μm. (N) qPCR results of Mdmx mRNA levels in vehicle and K-181 treated mice brains. N = 3.
