Figure 5
From: Tranilast protects pancreatic β-cells from palmitic acid-induced lipotoxicity via FoxO-1 inhibition
Protective effects of tranilast are associated with FoxO-1 inactivation. (A,B) INS-1 cells were treated with PA (500 μM) in the presence or absence of tranilast at the indicated concentrations for 24 h, and immunoblotting of whole cell lysates (A), or subcellular fractions (B, cytoplasmic and nucleus fractions) were carried out. Representative blots of three independent experiments are shown, and density analyses of western blots were presented in the lower panel. Original scans of whole blots are shown in Supplementary information. ##p < 0.01, ###p < 0.005 versus control, *p < 0.05, **p < 0.01, ***p < 0.005 versus PA alone.
