Figure 6
From: Tranilast protects pancreatic β-cells from palmitic acid-induced lipotoxicity via FoxO-1 inhibition
Effects of FoxO-1 S256A mutant on tranilast action. (A) Either FoxO-1 wild type (WT) or FoxO-1 mutant (S256A) was transfected into CHO cells, and the FoxO-1 localization after tranilast (100 μM) and PA (500 μM) treatment was observed using confocal microscopy. (B–D) INS-1 cells were transfected with either FoxO-1 WT or FoxO-1 mutant, and then treated with tranilast (100 μM) in the presence of PA for 24 h. Cell viability (B), DAPI staining (C) and western blot (D) were determined (scale bar = 20 μm). Original scans of whole blots are shown in Supplementary information. Data are expressed as mean ± SD of three independent experiments, each done in duplicate (B). Representative results are shown (A,C,D). ##p < 0.01, ###p < 0.005 versus control, ***p < 0.005 versus PA alone.
