Figure 5
The effect of DRP1i1 on mitochondrial morphology and cell survival. (A, B) The effect of DRP1i27 on promoting mitochondrial fusion in (A) human fibroblasts as well as (B) Drp1 wild type and KO MEF (n = 3–5). (C, D) The effect of DRP1i27 on (C) cell death and (D) percentage of cells with fragmented mitochondria in HL-1 cells subjected to simulated-ischaemia–reperfusion injury (n = 5–6).(E) The effect of DRP1i27 on lactate dehydrogenase (LDH) production in human iPSC-derived cardiomyocytes treated with DMSO (0.1%), DRP1i27 (50 µM), doxorubicin (DOX, 5 µM) or doxorubicin +DRP1i27 in combination (n=11 from 3 independent experiments). Data are expressed as mean ± SEM. *p ≤ 0.05 vs DMSO by one-way ANOVA with Bonferroni post hoc test.
