Figure 1 | Scientific Reports

Figure 1

From: The mitochondrial Ca2+ uniporter channel synergizes with fluid shear stress to induce mitochondrial Ca2+ oscillations

Figure 1

MCU OX causes [Ca2+]m transients/oscillations in sheared HUVECs. (A) A characteristic fluorescence image of mito-GCaMP6 shows overlap with the mitochondrial network, identified by mitoTracker Red, in an EC. (B) Characteristic mito-GCaMP6 microscope images of an EC monolayer at different times, while exposed to 1 min static followed by 9 min of SS. (C) Characteristic temporal profiles of normalized mito-GCaMP6 fluorescence for each EC in a microscope field of view, while exposed to either static incubation for 10 min (Static) or 1 min static incubation followed by 9 min of SS (SS). (D) A characteristic Western blot of lysates from untransduced, Ad.βGal- or Ad.MCU-transduced (all static) ECs against MCU, MICU2, and β-actin. Original/uncropped blots are presented in Supplementary Fig. 1 (15 µg total protein per lane). (E) Same as in (C), but for Ad.MCU-transduced ECs. (F) Magnification of a [Ca2+]m transient (dotted box in E). (G) Box plots of baseline fluorescence (F0) in arbitrary units and (H) of peak amplitude (ΔF) were plotted and statistically analyzed for 60 ECs (cells were from n = 4 independent experiments) in each condition tested; ns, P > 0.5.

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