Figure 5
The electrostatic profiles of the Lck-SH4-U domain as in the initial model (A), and according to clustering analyses after simulating the Lck-FL open state (B,C), and the Lck-FL closed state (D,E). Their electrostatic profiles were calculated in the ± 5 kT/e range and at pH 7.0 using the PDB2PQR38 and APBS39 tools. Electronegative and electropositive regions are indicated by the red and blue intensities respectively. The residues forming the anionic patch are shown as red sticks and labelled. PIP lipid binding residues (R39, R45) are shown as blue sticks for reference.
