Figure 2
From: Role of cardiac mitofusins in cardiac conduction following simulated ischemia–reperfusion
Functional characterization of isolated cardiomyocytes from mouse whole hearts using (OptioQUANT) platform. (A) Representative waveforms of action potential obtained from the wild-type (black trace) and Mfn1&2 KO (red traces) mouse cardiomyocytes. Dots represent AP points at 30%, 50%, 70% and 90% repolarization after depolarization. (B) Histograms (bottom) of wild type and mfn1&2 KO mouse cardiomyocytes show substantial variability and non-identical distribution of APD90. The empirical cumulative distribution plot (upper) shows prolonged action potential durations in the transgenic KO mouse cardiomyocytes. The blue dotted line illustrates median value. (C) Bar graph summarizing the APD at 30%, 50%, 70% and 90% of repolarization in WT (n = 77) and DKO (n = 152) cells. APD30: 0.015 ± 0.002 vs 0.015 ± 0.001; APD50: 0.024 ± 0.002 vs 0.033 ± 0.002; APD70: 0.044 ± 0.003 vs 0.066 ± 0.002 and APD90: 0.076 ± 0.004 vs 0.113 ± 0.003. All APD values in seconds. *p < 0.001 determined by Student's t-test. *eCDF: empirical cumulative distribution function; APD action potential duration.
