Figure 3

The impact of the novel disruption paradigm on RNA recovery. Total RNA (ng) was determined by computing the volume of the RNA elution buffer (38 µL) and the RNA concentration (ng/µL) measured via a Nanodrop Spectrophotometer at 260 nm. (a) Comparison of the total RNA extraction (per respective ILM) between the integrated method (experimental) (PCA, N = 6; LTA, N = 6; MTA, N = 6) relative to the manual and mechanical techniques (PCA, N = 6; LTA, N = 6; MTA, N = 6). (b) RIN values were obtained using different homogenization methods. The Agilent 2100 Bioanalyzer Pico Chip assay indicated the RNA stability of extracts. (c,f) Linear regression analysis correlating disruption time, tissue weight, and total RNA recovery from (c) PCA (m = 57.64, R² = 0.31) and (f) TA muscles (m = − 26.59, R² = 0.24). (d,g) Q-Q plot of the weight distribution in the PCA (d) and TA (g) muscles. (e,h) Residual analysis for validation of the linear regression in the PCA (e) and TA (h) muscles. Analysis of variance was performed using a two-way ANOVA with Posthoc tests. The data is expressed as mean ± SD. * means P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.