Figure 4
Setup of the apparatus that facilitated cryogenic disruption in RNase-free tubes as an alternative to tissue grinding via mortar and pestle. (a) Medium binder clips were attached to the lip of a surgical tray. Both metal wire arms of a complementary binder clip were wedged securely between the gripped section and the interior lip. (b) Liquid nitrogen (approx. 1 L) was poured directly into the tray. (c) BioMasherIII tubes containing the muscle fibers were stabilized by the looped arms of the attached clip. Once snap-frozen, tissues were crushed using disposable pestles. (d) Tissue grinding in liquid nitrogen ensued until fibers were finely broken down.
