Figure 5

The influence of adapted cryogenic disruption on total RNA recovery under experimental (+LN2) and standard conditions (−LN2). Data points plotted in dark blue signify standard conditions, or samples processed without liquid nitrogen (PCA, N = 15; LTA, N = 17; MTA, N = 16), while the light blue denotes experimental conditions, or the samples that were processed with liquid nitrogen (PCA, N = 20; LTA, N = 24; MTA, N = 22). The vertical lines associated with the respective colors represent the error, while the horizontal lines (dark black) indicate the mean. (a) Comparison of total nucleic acid extraction, determined by Nanodrop Spectrophotometer at 260 nm, under the experimental and standard conditions. (b) Changes in A₂₆₀/A_280, measured via Nanodrop, under experimental and standard conditions. (c) The impact of the RIN, provided by the Agilent 2100 Bioanalyzer Pico chip, due to cryogenic disruption under experimental and standard conditions. (d) Analysis of the tendencies of the 28S/18S ratios under experimental and standard conditions. Analysis of variance was performed using a two-way ANOVA with Posthoc tests. The data is expressed as mean ± SD. * means P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.