Figure 6
Validation of the pooling strategy. Total RNA was calculated from the volume of the RNA elution buffer (38 µL), and the RNA concentration (ng/µL) was measured via a Nanodrop Spectrophotometer at 260 nm. The black dashed lines denote the target yield of RNA for the different pooling strategies evaluated (200 ng). (a) Comparison of total RNA recovery from 1 pooled muscle (PCA, N = 6; LTA, N = 6; MTA, N = 6), 2 pooled muscles (PCA, N = 6; LTA, N = 6; MTA, N = 6), and 3 pooled muscles (PCA, N = 6; LTA, N = 6; MTA, N = 6). (b) Nested perspective showing the percentage of extracts greater than 200 ng (red). Analysis of variance was performed using a two-way ANOVA with Posthoc tests. The data is expressed as mean ± SD. * means P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
