Table 2 An overview of the specifications of the evaluated commercial kits and protocols.
QuickRNA microprep | RNeasy micro | TRIzol reagent | TRIzol + RNeasy micro | TRIzol + PureLink RNA mini | Aurum total RNA mini | |
|---|---|---|---|---|---|---|
Starting material | \(\le\) 10 mg tissue (stored at -80ºC) | < 5 mg tissue (stored at -80ºC) | 50–100 mg tissue (stored at -80ºC) | < 15 mg tissue (stored at – 80 °C) | \(\le\) 200 mg tissue (stored at – 80 °C) | \(\le\) 40 mg thawed tissue (stored at -80ºC) |
Approx. preparation time | ~ 20–30 min | ~ 30–40 min | ~ 1–2 h | ~ 1–2 h | ~ 60 min | ~ 30–60 min |
Binding capacity | 10 µg total RNA | 100 µg total RNA | N/A | 100 µg total RNA | 1000 µg total RNA | > 100 µg total RNA |
Storage | RT | RT | 15–30 °C | 15–30 °C/RT | 15–30 °C/RT | RT |
DNase digestion | Yes | Yes | No | No | No | Yes |
Elution volume | 20 µl | 10–14 µl | N/A | 20 µl | 30 µl | 30 µl-2 \(\times\) 40 µl |
Advantages | Rapid procedure | Rapid procedure | High RNA yield | High RNA yield | Reduced contamination | Purity > 1.96 |
Disadvantages | Inconsistent recovery | Inconsistent recovery | Low purity | Low purity | Low purity | Purity > 2.2 |
Low RNA yield | Low RNA yield | Phenol contamination | Phenol contamination | Phenol contamination | Low RNA yield |
