Table 8 Primers used for qPCR.
Gene | Forward primer | Reverse primer | Product size | Accession no |
|---|---|---|---|---|
FDPS | TCCTTCTGCCCATAATTCTCC | GGTGGTTCAGTGTCTGCTACG | 72 | NM_001253751.1 |
Otop1 | GCCAACAGCGTCCTGAATGA | TCCAAAACGATGGTGATGTTGC | 95 | NM_172709.3 |
PKD2L1 | CAGATGCGACAGGGACTGG | GGGGCTGTGTCCAACAGAC | 84 | NM_181422 |
PKD1L3 | TCCCACATCAGCTCAGAAGAAC | ATGGGGTGCTGTTCCATGTC | 141 | NM_001039700.3 |
CA4 | TACTGAAGACTCAGGCTGGTG | GGTCATAGCCGACGAGGATG | 179 | NM_007607.2 |
T1R3 | CAAGGCCTGCAGTGCACAA | AGGCCTTAGGTGGGCATAATAGGA | 92 | NM_031872 |
Gustducin | AGGGCATCTGAATACCAGCTCAA | CTGATCTCTGGCCACCTACATCAA | 196 | NM_001081143 |
ENaCα | GCTTCATCTTTACCTGTCGTTTC | CCAGAGATTGGAGTTGTTCTTGT | 122 | NM_001406028.1 |
Krt8 | TGAACAACAAGTTCGCCTCCTT | GCTCCTCGACGTCTTCTGCT | 110 | NM_031170 |
DSG1 | TCGTGGATGTAACAGAAGCCA | TGGCGTTATGATCTCTTACCTGG | 133 | NM_010079.2 |
DSG2 | GGGCCACTCACCATGTAAGG | TGTAGGAGGCCGCTTTCTCT | 162 | NM_007883.3 |
DSG3 | TCAGCCCGAAGACAAGGAAATC | TCTACCATGGTCCCACCAGC | 120 | NM_030596.4 |
Krt10 | GACAACTGACAATGCCAACG | GCCAGCTCTTCGTTCAGACT | 204 | NM_010660.2 |
Gapdh | TGTGTCCGTCGTGGATCTGA | TTGCTGTTGAAGTCGCAGGAG | 150 | NM_001289726 |
