Figure 3
From: Npp1 prevents external tooth root resorption by regulation of cervical cementum integrity
Npp1 suppresses β-catenin activity that drives the expression of non-collagenous proteins in Npp1-inactivated cementoblasts. (a) Protein levels were analyzed by Western blotting using specific antibodies with whole cell lysates from OCCM-30 cells with shEnpp1 and shNC harvested after differentiation with 10 mM β-GP treatment for indicated times. Samples shown in Western blotting are from the same experiment, and the gels/blots were processed under the same experimental conditions. β-Actin was used as a loading control. Cropped images are displayed here; the original full-size blots are presented in Supplementary Fig. S5 online. (b) The transcript levels of Bsp, Dmp1, and Opn were analyzed by real-time qPCR (n = 3). RNA was isolated from OCCM-30 cells transfected with mouse β-CatΔGsk as indicated and harvested after differentiation with 10 mM β-GP treatment for 4 days. (c) The transcript levels of Bsp, Dmp1, and Opn were analyzed by real-time qPCR (n = 3). RNA was isolated from OCCM-30 cells with shEnpp1 and shNC harvested after transfection with human GSK3β S9A (5 μg) for 24 h and differentiation with 10 mM β-GP treatment for 4 days. Significance was assigned with p-values as indicated in the graph.
