Figure 1
From: Transcriptomic and proteomic pathways of diabetic and non-diabetic mitochondrial transplantation
Experimental Model: ZDF rats (male, 14 weeks) were sedated and intubated. A left lateral thoracotomy was performed, and the heart was exposed. Regional ischemia was achieved by temporarily snaring the LAD. Following 30 min of ischemia, the snare was released, and 6 × 50 uL injections of vehicle solution alone (Vehicle) or vehicle solution containing mitochondria (1 × 106) isolated from either diabetic ZDF rat pectoralis major (diabetic ZDF-mitochondria) or mitochondria (1 × 106) isolated from non-diabetic Zucker lean rats (non-diabetic ZL-mitochondria) were delivered by epicardial injection into six pre-selected sites in the area-at risk, using a 1 mL tuberculin syringe with a 32-G needle. The ZDF rats were allowed to recover for 2 h or 28 days.
