Figure 5
From: Transcriptomic and proteomic pathways of diabetic and non-diabetic mitochondrial transplantation
Histology and multiplex analysis. Micrographs of ZDF rat hearts following 30 min. regional ischemia and 2 h (Hematoxylin and Eosin stained) (A) and 28 days (Masson’s tri-chrome stained) (B) recovery. Hearts receiving vehicle alone (Vehicle) show increased longitudinal and transverse interfibrillar separation as compared to hearts receiving diabetic ZDF -mitochondria and non-diabetic ZL -mitochondria. Representative transmission electron microscopy images (C) of ZDF rat hearts 28 days following 30 min of regional myocardial ischemia. Vehicle heart have swollen and electron-translucent mitochondria, with an enlarged intermembrane space, a disrupted matrix and calcium accumulation. Hearts that received either diabetic ZDF- or non-diabetic ZL-mitochondria show only traces of calcium accumulation in mitochondria and preserved mitochondrial structure. White arrows indicate calcium deposits. Magnification 16,000 x. All results are shown as the mean plus and minus the standard error of the mean for n = 6 for each group. Statistical differences are shown. Fibrosis area (D) calculated as the percentage of fibrotic tissue area (stained in blue) over the whole area of the myocardium per Masson’s trichrome slide. as *p < 0.05, **p < 0.01, ***p < 0.001; ns: not significant. Multiplex (42-plex) analysis of cytokines and chemokines using the Human Cytokine 42-plex Discovery Assay was performed for hearts following 30 min regional ischemia and 2 h (E) and 28 days (F) recovery for hearts receiving vehicle alone (Vehicle) or diabetic ZDF- or non-diabetic ZL -mitochondrial transplantation. Significantly increased cytokines as compared to vehicle. **p < 0.001. G: Heatmap showing peripheral blood cytokine analysis at 28 days recovery. The log fold change is shown with pseudocolor scale (−2 to 2) with blue denoting down-regulation and orange denoting up-regulation. Samples run in triplicates.
