Figure 6

Transcriptomic pathways following 30 min of regional myocardial ischemia and 2 h reperfusion. (A) Principal component analysis (PCA) for hearts receiving diabetic ZDF- (gold circles) or non-diabetic ZL -mitochondrial transplantation (blue circles) as compared to hearts receiving vehicle alone. (B) Heatmap of transcriptional regulation patterns following 30 min IRI and 2 h recovery, for hearts receiving diabetic ZDF- or non-diabetic ZL -mitochondrial transplantation. Fold changes (FC) are derived from comparison to Vehicle hearts. The log FC of gene expression is shown with pseudocolor scale (− 2 to 2), with blue denoting downregulation and orange denoting upregulation. Columns represent FC comparisons, and rows represent the genes. Experimental groups are found on the bottom. (C) A barplot summarizing the top downregulated and upregulated pathways. Colored barplots Red and blue, shown by arrows, indicate the pathways of interest. (D) Gene set enrichment analysis using MetaCore localization networks for hearts receiving diabetic ZDF- or non-diabetic ZL -mitochondria. (E) Gene set enrichment analysis using MetaCore biologic process analysis of non-diabetic ZL- as compared to diabetic ZDF- mitochondrial transplantation. Color coding indicates the gene ratio (orange higher-blue lower) and size of the dot indicates the number of the genes. Gene network visualization of (F) “Skeletal muscle development” and (G) “Cardiac development” pathways showing a central node of troponins. Node sizes are proportional to the number of dataset proteins present in that pathway node. Node color and scale bars depict the level of betweenness centrality for that pathway node.