Figure 1
From: Direct encapsulation of biomolecules in semi-permeable microcapsules produced with double-emulsions
Production of semipermeable microcapsules in a PDMS device with 3D geometry. (A) Schematic representation of the PDMS device with 3D geometry. W/O/W double-emulsions are generated with a PEG-DA 258 middle phase encapsulating an inner aqueous core. (B) Micrographs of the PDMS device operation. (C) Brightfield image of microcapsules obtained after UV polymerization of the collected double-emulsion. (D) Size distribution of a representative batch of polymerized microcapsules. (E) Schematic representation of PIPS upon UV illumination. 15% Butyl-acetate (porogen) was mixed with PEG-DA 258 to form semi-permeable microcapsules. (F) In the collected double-emulsion, both high molecular weight 500 kDa FITC-dextran and lower molecular weight 40 kDa RITC-dextran are retained in the inner aqueous phase. (G) After UV polymerization and PIPS, pores are formed in the capsule shell and capsules become semi-permeable.
