Figure 4
From: Direct encapsulation of biomolecules in semi-permeable microcapsules produced with double-emulsions
Direct encapsulation of functional enzymes in semi-permeable PEG-DA 258 microcapsules. A luciferase-GFP fusion protein was directly encapsulated in semi-permeable capsules. The fluorescent fusion protein allows for the visualization of the enzyme (A) in the double-emulsion, and (B), in the polymerized capsules. (C) The encapsulated econoLuciferase shows a strong signal in a bioluminescent assay. Direct encapsulation of \(\beta \)-galactosidase. (D,E) Enzyme-containing capsules were dispersed in trehalose and air-dried at 37 \(^{\circ }\)C. (F) After rehydration with a solution containing CPRG, the substrate was hydrolyzed to chlorophenol red. (G) The solution was imaged with a color camera mounted on an inverted microscope with \(\times \) 4 magnification. Capsules displayed a purple color in their interior, indicative of \(\beta \)-galactosidase enzymatic activity.
