Figure 2 | Scientific Reports

Figure 2

From: Humoral and cellular immune responses to Lassa fever virus in Lassa fever survivors and their exposed contacts in Southern Nigeria

Figure 2

Binding antibody responses (IgG and IgM) to LASV GP and NP proteins (a) and neutralizing antibody response to LASV PVs (Lineages I-V) (b) from LF survivors and their contacts in Southern Nigeria. Specific Lassa GP and NP ELISA was used to measure the binding antibody response (IgG and IgM) from LF survivors (N = 370) and their contacts (N = 170). We used a 1:100 dilution of the serum sample. Among those with a binding antibody response, we selected some sera from LF survivors and their contacts and measured the neutralizing titre (IC50) against LASV PV expressing the LASV GPC of different lineages ((Lineages I–V). The OD of the negative cutoff was selected as the mean multiplied by three standard deviations of three known negative samples (0.25) for binding antibody and 40 for neutralizing antibody response (the limit of detection). The table shows geometric mean at 95% CI. Statistical significance was calculated by Mann–Whitney test and p values are indicated. (Capped line with * indicating significance).

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