Figure 1

Response surface plots (left) and contour plots (right) of salinity and temperature to digestive enzyme activities and hepatopancreas immune gene relative expression of P. fucata. The activities of AMS, LPS, PEP and TRYP, as well as the relative expression levels of GPX and SOD genes in hepatopancreas were shown from top to bottom. The heat map in Fig. 1 was generated using Design Expert (https://www.statease.com/software/design-expert/, version 10.0.7.0 32-bit) software.