Figure 2

Response surface (left) and contour map (right) of salinity and temperature to respiratory metabolic enzyme activities and gill immune gene relative expression of P. fucata. The activities of LDH, NKA and AKP, and the relative expression levels of GPX and SOD genes in gills were shown from top to bottom. The heat map in Fig. 2 was generated using Design Expert (https://www.statease.com/software/design-expert/, version 10.0.7.0 32-bit) software.