Table 6 Experimental design and results of digestive response surface.

From: The combined effects of temperature and salinity on the digestion and respiration metabolism of Pinctada fucata

Run

Coded

Actual

AMS

LPS

PEP

TRYP

GPX-hepatopancreas

SOD-hepatopancreas

T(℃)

S (‰)

T(℃)

S (‰)

(AU/mgprot)

(AU/gprot)

(AU/mgprot)

(AU/mgprot)

–

–

1

0

1

25

33

0.89

2.132

2.61

712.33

0.853

0.5369

2

−1

0

20

28

0.86

2.101

2.51

815.34

0.483

2.8700

3

0

0

25

28

1.19

2.131

2.62

849.45

0.612

0.4369

4

−1

−1

20

23

0.27

2.107

2.47

719.76

1.020

7.5500

5

0

1

25

33

0.89

2.135

2.65

699.35

0.833

0.5030

6

0

0

25

28

1.02

2.120

2.63

855.48

0.632

0.4769

7

1

−1

30

23

0.97

2.154

2.79

692.74

0.993

0.7753

8

−1

0

20

28

0.86

2.110

2.51

812.22

0.483

0.8277

9

0

−1

25

23

0.77

2.120

2.62

747.35

1.075

2.8700

10

0

0

25

28

1.14

2.131

2.63

864.28

0.603

0.4469

11

1

0

30

28

0.92

2.160

2.76

812.35

1.009

0.5300

12

0

0

25

28

1.17

2.135

2.68

868.34

0.632

0.3069

13

1

1

30

33

0.58

2.163

2.59

758.78

1.431

1.8300

14

0

−1

25

23

0.77

2.113

2.66

761.84

1.015

2.8700

15

−1

1

20

33

0.86

2.119

2.59

645.35

0.503

1.3800

16

0

0

25

28

1.07

2.135

2.62

855.74

0.692

0.4469

17

1

0

30

28

0.92

2.159

2.71

880.82

1.251

0.5030

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