Table 2 Primers and probes for qPCR quantification of stx2d55.

From: Impact of Shiga-toxin encoding gene transduction from O80:H2 Shiga toxigenic Escherichia coli (STEC) on non-STEC strains

Gene

Primer or probe

Sequence (5′–3′)

Position (5′–3′)

Accession number

stx2

stx2-F

TCA GGC AIA TAC AGA GAG AAT TTC G

578–602

AY443044

stx2-Ra

CCG GIG TCA TCG TAT ACA CAG

646–626

AY443044

stx2-PΦ

CAC TGT CTG AAA CTG CT

608–624

AY443044

  1. Ф Probe tagged with minor groove-binding non-fluorescent quencher (MGBNFQ) and 6-carboxyfluorescin (FAM) fluorescent label (Eurogentec).
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