Figure 2 | Scientific Reports

Figure 2

From: Hypoxia, acidification and oxidative stress in cells cultured at large distances from an oxygen source

Figure 2

Measurement of metabolic parameters in HaCaT parental and HaCaT E5/E6/E7-18 cell lines. (A) Oxygen Consumption Rate (OCR) (B) Extracellular Acidification Rate (ECAR) (C) Lactate production levels measured in HaCaT parental and HaCaT E5/E6/E7–18 cells in normoxia and hypoxia conditions. (D) CO2 levels measured in HaCaT parental and HaCaT E5/E6/E7-18 cells in normoxia and hypoxia conditions. (E) pH measurements in HaCaT parental and HaCaT E5/E6/E7–18 cells in normoxia and hypoxia. (F) Changes in phenol red culture medium can be seen after coverslip treatment. (*) P < 0.05, (**) P < 0.01, (***) P < 0.001.

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